Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish

Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish

Blog Article

Allogeneic here organ transplantation is a powerful tool for clinical and basic research studies.However, the graft is often rejected by the host organism.Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish.These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an allogeneic testis and testicular hyperplasia.

This protocol can be used to amplify and maintain testicular hyperplasia grafts for several years (Kawasaki et al., 2016).The here amplified hyperplasias are likely to be a good source of somatic and germ cells such as Sertoli cells and spermatogonial stem cells.